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Article

  • Title

    Development of method for the mineral water catalase activity determination

  • Authors

    Nikipelova Е. М.
    Kisilevskaya А. Yu.
    Solodova L. B.

  • Subject

    CHEMISTRY. CHEMICAL ENGINEERING

  • Year 2015
    Issue 1(45)
    UDC 615.327.07
    DOI 10.15276/opu.1.45.2015.29
    Pages 176-180
  • Abstract

    Biological effects of mineral water depend not only on the chemical composition but also on the metabolic products of microbial cenosis. Among numerous microorganisms constituting the autochthonous microflora of mineral waters, we do evolve the saprophytic organisms producing the catalase, the saprophytes’ physiological and biological role being proven a long ago. The research aim was to develop a method for determination of mineral water catalase activity. Analysed are various methods to determine the catalase activity in biological objects. Developed is a spectrophotometric method for determination of mineral water catalase activity. The method is efficiently tested with series of Ukrainian mineral waters. Calculated are the relative standard deviations which are significantly below normal errors, admitted at spectroscopic analysis and at the optic density range. The given method provides sufficient accuracy and convergence when estimating the mineral waters catalase activity, allowing to introduce a new index to assess the quality and biological value.

  • Keywords catalase activity, mineral waters, spectrophotometric method, saprophytic microorganisms
  • Viewed: 852 Dowloaded: 5
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  • References

    Література
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    6.    Wu, M., Lin, Z., & Wolfbeis, O.S. (2003). Determination of the activity of catalase using a europium(III)–tetracycline-derived fluorescent substrate. Analytical Biochemistry, 320(1), 129—135.
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    9.    Sippy, N., Luxton, R., Lewis, R.J., & Cowell, D.C. (2003). Rapid electrochemical detection and identification of catalase positive micro-organisms. Biosensors and Bioelectronics, 18(5–6), 741—749.
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